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KMID : 0613820190290030332
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Journal of Life Science
2019 Volume.29 No. 3 p.332 ~ p.336
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Inhibitory Effects of Chrysanthemum boreale Makino on 3T3-L1 Preadipocyte Differentiation and Down-regulation of Adipogenesis and Lipogenesis
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Hwang Dae-Il
Choi In-Ho
Kim Do-Yoon
Park Soo-Min
Kim Ha-Bin
Li Ya-Li
Lee Hwan-Myung
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Abstract
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Obesity is associated with an increased risk of many diseases including type 2 diabetes mellitus, hypertension, and hyperlipidemia. The flowers of Chrysanthemum boreale have been used as traditional medicines for the treatment of diseases such as obesity and type 2 diabetes mellitus. This study aimed to evaluate the effect of C. boreale Makino flower essential oil (CFEO) on adipocyte differentiation using preadipocyte cell line 3T3-L1. CFEO at concentrations between 0.1 and 5 μg/ml did not affect 3T3- L1 cell viability. A CFEO concentration of between 0.1 and 1 μg/ml significantly inhibited lipid accumulation during MDI-induced differentiation in 3T3-L1 cells in a dose-dependent manner, reaching a maximal level at 1 μg/ml (28.94±2.01%; approximately 30% of control treated with MDI alone). Western blot analysis revealed that CFEO concentrations between 0.1 and 1 μg/ ml suppressed the activations of three adipogenic transcription factors in the MDI-stimulated 3T3-L1 cells: peroxisome proliferator-activated receptor γ; CCATT/enhancer binding protein α; and sterol regulatory element binding protein-1. Moreover, the expressions of lipogenic enzymes, acetyl-CoA carboxylase, and fatty acid synthase were also inhibited by treatment with CFEO between 0.1 and 1 μg/ml. CFEO may therefore be a promising functional material for obesity prevention.
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KEYWORD
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3T3-L1 cells, adipogenesis, Chrysanthemum boreale Makino, essential oil, obesity
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